Regulation of Termitomyces clypeatus protoplast regeneration by Krebs cycle acids
نویسندگان
چکیده
منابع مشابه
Thin layer chromatography of Krebs cycle acids.
A systematic appraisal of a large number of solvent systems was carried out for the separation of lactic, pyruvic and 3-hydroxybutyric acids and all of the KREBS cycle acids, except oxalosuccinic, using silica gel G and cellulose thin layer Chromatographie plates. Four solvent systems which allowed separation of various combinations of acids are described and the limits of this method of separa...
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Krebs cycle enzyme activity in Bacillus subtilis was examined under aerobic and anaerobic conditions. Citrate synthase and aconitase activities in cells grown anaerobically in the presence of nitrate were reduced by as much as 10- and 30-fold, respectively, from levels observed under aerobic culture conditions. The maximum level of isocitrate dehydrogenase activity during anaerobic growth was o...
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Production profiles and characterization of the sucrase-cellobiase (S-C) co-aggregates from the filamentous fungus Termitomyces clypeatus were compared in media containing 1% and 5% sucrose to understand the effect of cellular regulation over secretion and aggregation of these two industrially important glycosidases. The enzymes were secreted constitutively but in a high sucrose medium (5%), ce...
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The high osmotic pressure provided for in vitro transformation processes to support naked protoplasts of Saccharomyces cerevisiae was found to hurt regeneration of the protoplasts to walled cells. Preadaptation of the cells by growth under the same hypertonic conditions as those used for transformation doubled the rate of protoplast regeneration and in turn resulted in a higher yield of transfo...
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Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described.
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097
DOI: 10.1016/0378-1097(91)90206-p